Combined positive effect of oocyte extracts and brilliant cresyl blue stained recipient cytoplasts on epigenetic reprogramming and gene expression in buffalo nuclear transfer embryos
نویسندگان
چکیده
منابع مشابه
the effect of melatonin on maturation, glutathione level and expression of hmgb1 gene in brilliant cresyl blue (bcb) stained immature oocyte
objective: nutrients and antioxidants in the medium of immature oocyte have a profound effect on maturation, fertilization and development of resulting embryos. in this study the effects of melatonin as an antioxidant agent on maturation, glutathione level and expression of high mobility group box-1 (hmgb1) gene were evaluated in immature oocytes of mice stained with brilliant cresyl blue (bcb)...
متن کاملThe Effect of Melatonin on Maturation, Glutathione Level and Expression of H MGB1 Gene in Brilliant Cresyl Blue (BCB) Stained Immature Oocyte
OBJECTIVE Nutrients and antioxidants in the medium of immature oocyte have a profound effect on maturation, fertilization and development of resulting embryos. In this study the effects of melatonin as an antioxidant agent on maturation, glutathione level and expression of High mobility group box-1 (HMGB1) gene were evaluated in immature oocytes of mice stained with brilliant cresyl blue (BCB)....
متن کاملP-100: Improved Efficiency of Somatic Cell Nuclear Transfer in Sheep by Persecution of Recipient Oocytes with Brilliant Cresyl Blue Staining
Background: The most important factor affecting the efficiency of somatic cell nuclear transfer (SCNT) is the initial quality of the oocyte. Since the abattoir-derived oocytes are heterogeneous in quality, selection of cohort fully grown/competent oocytes is necessary for proper development of SCNT embryos. Materials and Methods: Glucose-6-phosphate dehydrogenase (G6PDH) is actively expressed i...
متن کاملBrilliant Cresyl Blue in Sap of Nitella
It has been shown by several investigators I that when the pl-I value of the external dye solutions is increased, the rate of accumulation of a basic dye in the cell sap is accelerated. Two different explanations for this have been given. Some have accounted for it on the basis that the basic dye enters the cell in the form of a dye hydrate and combines with the substances in the cell; and that...
متن کاملEpigenetic reprogramming and nuclear transfer
Nuclear transfer, also referred to as cloning, attracted public attention when the first mammal, the sheep Dolly, was cloned from an adult cell. In this review, we highlight some of the milestones reached in the 50 years following the first nuclear transplantations in frogs. We also address the problems associated with nuclear transfer and provide an survey on current stem-cell technology. In t...
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ژورنال
عنوان ژورنال: Cytotechnology
سال: 2017
ISSN: 0920-9069,1573-0778
DOI: 10.1007/s10616-016-0057-0